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. 2018 Mar 22;103(6):939–948. doi: 10.3324/haematol.2017.181354

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Figure 2. — JQ1 induces T-cell apoptosis and inhibits B-cell maturation. Animals received daily intraperitoneal (i.p.) injections of 50 mg/kg JQ1 for 21 days after which the different parameters were analyzed. (A–C) Flowcytometric quantification of Pro-B (A), Pre-B (B) and immature B cells to analyze B-cell development in bone marrow (BM) (n=4; *P<0.05). (D–F) Flowcytometric quantification of CD4⁻CD8⁻ double negative (D), CD4⁺CD8⁺ double positive (E) and all differentiation states of CD4⁻CD8⁻ double negative T cells (DN1-4) in thymus (n=4; *P<0.05). (G) Flowcytometric quantification of apoptosis in CD4⁻CD8⁻ double negative, CD4⁺CD8⁺ double positive and CD4/CD8 single positive T cells using Annexin V (n=3; *P<0.05). (H) Flowcytometric quantification of apoptosis in pro-B, pre-B, immature-B (Imm) and mature B cells using Annexin V (n=4; *P<0.05). (I) FACS-sorted T-cell subpopulations from thymus of placebo or JQ1-treated mice were analyzed for mRNA expression via qRT-PCR (n=5; *P<0.05).