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. 2018 Mar 22;103(6):939–948. doi: 10.3324/haematol.2017.181354

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Figure 3. — JQ1 treatment results in expansion and mobilization of hematopoietic stem cells (HSC). Animals received daily intraperitoneal (i.p.) injections of 50 mg/kg JQ1 for 21 days after which the different parameters were analyzed. (A) Different FACS-sorted hematopoietic stem and progenitor cells from bone marrow (BM) of mice were analyzed for baseline mRNA expression of Brd4 via qRT-PCR (n=2–3; *P<0.05). (B–D) Flowcytometric quantification of phenotypic lineage-ckit⁺sca1⁺ cells (LSK) (B), ST-HSC and LT-HSC (C), as well as MPP (D) in BM (n=5–9; *P<0.05). (E–G) Colony formation assays to quantify hematopoietic progenitors in BM (E), spleen (F), and peripheral blood (G) (n=3–8; *P<0.05). (H) Replating of CFU from BM (n=3; *P<0.05). (I) Flowcytometric quantification of BrdU incorporation in LT-HSC in BM (n=5; *P<0.05). (J) Flowcytometric quantification of apoptosis in LT-HSC in BM using Annexin V (n=5; *P<0.05).