Figure 5.

Immunohistologic Staining for vWF⁺ Cells in Silk Grafts at Explantation

Representative micrograph images of von Willebrand factor (vWF) immunohistochemistry staining on cross sections of silk grafts at 3 (A), 6 (B), 12 (C), and 24 (D) weeks. vWF staining is red, and 4′,6-diamidino-2-phenylindole staining is blue. Scale bar = 200 μm. (E) Quantification of vWF coverage on the lumen of the graft depicting vWF positivity as early as 3 weeks and complete coverage by 12 weeks. N = 4 animals per time point with 3 images analyzed per region per graft. The red dashed line indicates the average vWF⁺ staining for each time point. Statistical analysis was performed on these values. D → P indicates selected equidistant regions within the graft, from distal to proximal.