Figure 3.

Enhanced ABCC4 Protein Expression in HIV

(A) Immunoblots of ABCC4 in platelet lysates from controls (C) (n = 8) and HIV (n = 14) subjects. Thirty micrograms of protein were loaded, and GAPDH was used as a loading control. Quantification after normalization is reported. *p < 0.05. (B) Fluorescence microscopy showing ABCC4 expression (green) and f-actin (red) in HIV (n = 3) and controls (n = 3). Image is representative of a single platelet for each group. The quantification reports ABCC4 green dots/cell counted in 3 subjects per group (>50 cells for each sample). *p<0.05. Scale bar: 5 μm. GAPDH = glyceraldehyde 3-phoshate dehydrogenase; other abbreviations as in Figure 1.