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. 2018 Jul 18;12:146. doi: 10.3389/fnbeh.2018.00146

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Copyright © 2018 Roccaro-Waldmeyer, Girard, Milani, Vannoni, Prétôt, Wolfer and Celio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunfluorescence staining with the VGlut-2 antiserum. The two upper nanozoomer images (A,B) show a low magnification of the periaqueductal gray, with the Su3-region demarcated on the right in CTR (A; F31) and KO (B; F33) mice. The Su3 receives inputs from the PV/VGlut2 neurons of the parvafox nucleus of the lateral hypothalamus and we supposed that a difference in density of terminals could be recognized at the light microscopic level. This is indeed the case, the immunolabelling for VGlut2-terminals being more intensive in the CTR (C and terminal density in the inset) than in the KO-Su3 (D and terminal density in the inset). Aq, aqueduct; mlf, medial longitudinal fasciculus; PAG, periaqueductal gray; Su3, supraoculomotor nucleus; Su3C, supraoculomotor cap. The asterisks in the insets indicate the approximate region in (C,D), from which the high magnification image was obtained.