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. Author manuscript; available in PMC: 2018 Nov 2.
Published in final edited form as: Nature. 2018 May 2;557(7705):381–386. doi: 10.1038/s41586-018-0079-1

Figure 1. Discrete mode of GPCR-activated cellular β-arrestin trafficking is broadly conserved.

Figure 1

(a–d) Live cell TIRF microscopy images showing (a) FLAG–β1AR (blue) or (c) FLAG–β2AR (blue), β-arrestin-2–GFP (green) and clathrin-light-chain–DsRed (red) before and after 10 μM isoproterenol treatment. Average enrichment at CCSs after 10 μM isoproterenol treatment for (b) FLAG–β1AR (d) FLAG–β2AR (n=14 and 15 cells, respectively, from 3 independent experiments, data shown as mean ± s.e.m.). (e) Live cell TIRF microscopy images of HEK 293 cells co-expressing super ecliptic pHluorin–β2AR (blue), β-arrestin-2–mApple (green), and clathrin-light-chain–TagBFP (red) before and after 10 μM isoproterenol treatment. (f) Timelapse of individual pre-existing CCSs from panel e. Scale bars, 5 μm. (a, c, e, f) show representative images from 3 independent experiments.