Fig. 4.

—(A) Schematic representation of TEV HC-Pro and CMV 2b viral suppressors of RNA silencing proteins as present in the engineered TEV-FINK/2b and TEV-AS13/2b genomes. The main structural and functional region/domains and relevant motifs of HC-Pro and 2b are indicated. RSS: RNA silencing-suppression region. RBD: RNA-binding domains within the RSS. CPD: cysteine proteinase domain. Position of the TEV FRNK and ED amino acid motifs indicated with a horizontal line below the corresponding RBD regions. Position of the C345 and H418 dyad amino acids of the catalytic CPD in the Ct of the central region, are indicated by an asterisk. (B) Ancestral virus genotypes used in this work with the relevant amino acids and 2b insertion marked by a gray box. (C) Same as in panel (B) but indicating the mutations found in the two genes in evolving viral lineages after one (P1) and five (P5) passages. The position and type of additional mutations found are marked with triangles. Large triangles denote amino acid replacements fixed in the consensus sequence (>50%) while small ones represent minority viral subpopulations. For additional details, see supplementary table S1, Supplementary Material online.