Table 2.
Elaborate physicochemical characterization of source materials and substrates used.
| pH | Moisture | Total nitrogen | Protein | Ash | Organic matter | C/N ratio | Crude fibre | Crude fat | NFE | Cellulose | NDS | ||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Base materials |
WS | 5.85 | 685 | 3.5 | 21.9 | 76.0 | 924.0 | 153.1 | 391.4 | 6.9 | 503.8 | 407.8 | 156.8 |
| SPS | 5.50 | 689 | 4.8 | 30.0 | 89.0 | 911.0 | 110.1 | 190.2 | 6.6 | 684.2 | 453.1 | 166.0 | |
| WB | 6.64 | 112 | 23.9 | 149.4 | 61.8 | 938.2 | 22.8 | 137.3 | 30.0 | 621.5 | 134.1 | 353.8 | |
| Substrate made |
T1 | 7.70 | 713 | 3.6 | 22.5 | 199.3 | 800.8 | 129.0 | 355.8 | 5.8 | 416.6 | 324.5 | 156.3 |
| T2 | 8.09 | 733 | 7.3 | 45.3 | 226.3 | 773.8 | 61.9 | 334.2 | 10.4 | 383.8 | 295.0 | 165.6 | |
| T3 | 8.36 | 743 | 11.6 | 72.6 | 241.1 | 758.9 | 37.9 | 306.6 | 13.8 | 365.9 | 262.8 | 183.7 | |
| T4 | 7.11 | 714 | 16.4 | 102.4 | 218.9 | 781.1 | 27.7 | 261.4 | 16.5 | 400.9 | 225.4 | 230.4 | |
| T5 | 7.37 | 711 | 4.7 | 29.6 | 247.3 | 752.7 | 92.2 | 322.0 | 5.5 | 395.6 | 299.1 | 186.1 | |
| T6 | 8.16 | 725 | 8.0 | 49.7 | 282.5 | 717.5 | 52.3 | 301.4 | 10.2 | 356.3 | 289.4 | 166.1 | |
| T7 | 8.28 | 739 | 11.2 | 70.2 | 230.6 | 769.4 | 39.7 | 287.3 | 13.6 | 398.3 | 270.1 | 200.1 | |
| T8 | 8.03 | 713 | 13.7 | 85.7 | 268.7 | 731.4 | 30.9 | 264.9 | 16.2 | 364.6 | 227.6 | 210.5 | |
| T9 | 7.19 | 704 | 4.0 | 25.0 | 281.6 | 718.4 | 104.2 | 320.0 | 5.3 | 368.2 | 309.0 | 147.8 | |
| T10 | 7.81 | 718 | 7.8 | 48.6 | 234.5 | 765.6 | 57.0 | 322.6 | 9.9 | 384.4 | 317.9 | 167.9 | |
| T11 | 8.26 | 709 | 10.3 | 64.6 | 267.6 | 732.4 | 41.1 | 294.5 | 13.3 | 359.9 | 268.5 | 179.3 | |
| T12 | 8.17 | 715 | 12.7 | 79.2 | 252.1 | 747.9 | 34.2 | 276.7 | 16.0 | 376.1 | 248.8 | 209.1 | |
| T13 | 8.08 | 735 | 8.2 | 51.1 | 70.6 | 929.4 | 65.9 | 448.4 | 14.1 | 415.7 | 389.1 | 181.3 | |
| T14 | 7.94 | 711 | 8.0 | 49.9 | 95.1 | 904.9 | 65.7 | 404.7 | 12.9 | 437.4 | 383.1 | 169.7 | |
| Average | 7.90 | 720.21 | 9.1 | 56.9 | 222.6 | 777.4 | 59.9 | 321.5 | 11.7 | 387.4 | 293.6 | 182.4 | |
| CV (%) | 5.17 | 1.73 | 41.2 | 41.3 | 28.7 | 8.2 | 50.0 | 16.3 | 33.7 | 6.3 | 16.9 | 12.7 | |
WS, wheat straw; SPS, spent Pleurotus ostreatus (Jacq.) P. Kumm. substrate; WB, wheat bran; T, treatment; T1, WS 6.000 g; T2, WS 5.400 g + WB 600 g; T3, WS 4.800 g + WB 1.200 g; T4, WS 4.200 g + WB 1.800 g; T5, WS 3.000 g + SPS 3.000 g + CaCO3 60 g; T6, WS 2.700 g + SPS 2.700 g + WB 600 g + CaCO3 54 g; T7, WS 2.400 g + SPS 2.400 g + WB 1.200 g + CaCO3 48 g; T8, WS 2.100 g + SPS 2.100 g + WB 1.800 g + CaCO3 42 g; T9, SPS 6.000 g + CaCO3 120 g; T10, SPS 5.400 g + WB 600 g + CaCO3 108 g; T11, SPS 4.800 g + WB 1.200 g + CaCO3 96 g; T12, SPS 4.200 g + WB 1.800 g + CaCO3 84 g; T13, commercially controlled based substrates (Quintanar del Rey); T14, commercially controlled based substrates (Villamalea). CV, coefficient of variation; NFE, nitrogen free extractives; NDS, neutral detergent-soluble. Results expressed in g/kg dry matter, except pH, moisture (fresh matter) and C/N ratio.