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. 2017 Feb 10;109(6):djw297. doi: 10.1093/jnci/djw297

Figure 3.

Figure 3.

Anaphase catastrophe in lung cancer cells after CCT68127 treatment. A) Comparison of CCT68127 effects on growth of lung cancer cells between vehicle controls, washout (CCT68127 washout after 48 hours’ treatment), and CCT68127 (continuously treated) groups. B) Percentages of cells undergoing multipolar anaphase after CCT68127 treatment in murine (ED1 and LKR13) and human (A549 and Hop62) lung cancer cells, and bipolar immortalized (C10 and Beas-2B) cells. Representative ED1 lung cancer cells are displayed in the upper panels with two spindle poles (vehicle control) and for those undergoing multipolar anaphases (four spindle poles in the middle panel and three spindle poles in the right panel) in the presence of CCT68127 treatment. The blue signal is DAPI staining, and the red signal is α-tubulin staining. Scale bars = 5 µm. C) Effects of engineered CP110 overexpression on growth inhibition by CCT68127 treatment of lung cancer cells. Error bars are standard deviation. The P values were computed using t test (C) with multiple comparison adjustment by the Tukey's method (A) and Dunnet's method (B). All statistical tests were two-sided.