Figure 4.

Involvement of PEA15 in CCT68127 antineoplastic effects in lung cancer cells. A) Heatmaps of protein expression profiles independently analyzed by RPPA in murine (ED1 and LKR13) and human (H522 and Hop62) lung cancer cells after vehicle or CCT68127 treatments. Clusters of proteins markedly downregulated by CCT68127 treatment are displayed. Complete heatmaps appear in Supplementary Figure 4 (available online). B) Quantifications of PEA15 and PEA15-pS116 protein levels by RPPA after CCT68127 vs vehicle treatments are shown with error bars representing standard deviation. C) Consequences of engineered gain of PEA15 expression on proliferation of CCT68127-treated lung cancer cells. Immunoblot analyses confirmed PEA15 overexpression in upper panels. Error bars show standard deviations. The P values were computed by t tests. All statistical tests were two-sided.