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. 2018 Jul 25;13(7):e0200207. doi: 10.1371/journal.pone.0200207

Fig 4. Small RNA Northern blot analyses of selected miRNAs.

Fig 4

Expression of the conservative (green) and the newly predicted (red) miRNAs. The total RNA samples from the seed, the placenta and the flesh at 28 and 40 days after anthesis (DAA) were run on agarose gels, transferred to nylon membranes, and hybridized with probes detecting miRNAs as indicated. We show the NGS read counts (read/million) in the upper panels, the results of small RNA northern blots in the middle panels, and the rRNA levels as loading controls in the lower panels. We used the same membrane for the hybridization of miR171, miR396, and miR159, can-mir-f13 specific probes.