Fig 8. Brain sequestered macrophages preferentially express Vβ3, Vβ7, and Vβ8.1/2 during experimental cerebral malaria.

A: C57BL/6 mice were infected with 10⁶ Plasmodium berghei ANKA parasites and T cell receptor Vβ usage was evaluated in splenic CD11b^highCD14⁺F4/80⁺ macrophages of moribund mice (n = 5) on day 6 post-infection using a flow cytometry based mouse Vβ TCR Screening Panel. B: The type of and preference for Vβ TCR was also assessed in brain sequestered CD11b^highCD14⁺F4/80⁺ macrophages during the cerebral phase of experimental cerebral malaria. Results shown depict the proportion of macrophages that express each Vβ chain and the total number of macrophages that express each Vβ chain after normalization for cell volume and are representative of three independent experiments.