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. 2018 Jul 13;14(7):e1007517. doi: 10.1371/journal.pgen.1007517

Fig 2. Mutagenesis method.

Fig 2

(A) The Sup35 prion domain contains an N-terminal Q/N-rich prion nucleation domain, and an oligopeptide repeat domain. The nucleation domain in full-length Sup35 was replaced with the core PrLDs from hnRNPA1 and hnRNPA2. An 8-amino-acid segment in each of the fusion proteins was then randomly mutagenized. Mutants were expressed as the sole copy of Sup35 in the cell. Library members were screened for their initial adenine phenotype, and for the ability to form prions. (B) Sequences of the core PrLDs from hnRNPA1 and A2, and the nucleation domain of Sup35. The underlined segments of hnRNPA1 and A2 were mutagenized in this study, while the underlined segment of Sup35 was mutagenized previously [44]. Arrow heads indicate the sites of hydrophobic insertion in Figs 7A and 6B.