Table 1. Amino acid representation among ADE⁺ and ade^- isolates.

Amino Acids	hnRNPA2-Sup35 Fusion Library				hnRNPA1-Sup35 Fusion Library
	Frequency		ln(ORA) ǂ	P value	Frequency		ln(ORA) ǂ	P value
	ADE+ Library	ade- Library			ADE+ Library	ade- Library
Valine	0.078	0.023	1.30	7.8 x 10−4	0.083	0.042	0.74	0.049
Methionine	0.041	0.013	1.21	0.024	0.033	0.011	1.12	0.074
Leucine	0.125	0.045	1.11	1.7 x 10−4	0.100	0.042	0.94	6.3 x 10−3
Isoleucine	0.047	0.020	0.89	0.049	0.083	0.033	0.97	9.2 x 10−3
Tyrosine	0.057	0.040	0.38	0.29	0.038	0.031	0.21	0.65
Alanine	0.051	0.040	0.25	0.58	0.038	0.036	0.039	1.00
Phenylalanine	0.047	0.040	0.18	0.71	0.067	0.042	0.50	0.19
Proline	0.057	0.053	0.10	0.87	0.063	0.064	-0.023	1.00
Threonine	0.057	0.055	0.046	1.00	0.054	0.061	-0.13	0.86
Serine	0.128	0.125	0.031	0.91	0.125	0.119	0.052	0.90
Tryptophan	0.024	0.025	-0.057	1.00	0.033	0.025	0.30	0.62
Aspartic Acid	0.041	0.053	-0.27	0.59	0.021	0.047	-0.85	0.12
Glycine	0.068	0.088	-0.28	0.39	0.033	0.075	-0.85	0.034
Histidine	0.034	0.045	-0.30	0.56	0.050	0.042	0.19	0.69
Lysine	0.020	0.028	-0.31	0.63	0.004	0.033	-2.11	0.019
Cysteine	0.030	0.050	-0.52	0.25	0.063	0.036	0.58	0.17
Arginine	0.054	0.110	-0.77	9.4 x 10−3	0.058	0.125	-0.84	7.6 x 10−3
Glutamic Acid	0.014	0.030	-0.81	0.20	0.017	0.019	-0.16	1.00
Glutamine	0.014	0.035	-0.97	0.093	0.017	0.039	-0.87	0.15
Asparagine	0.014	0.085	-1.91	1.5 x 10−5	0.021	0.078	-1.38	2.9 x 10−3
Groups of amino acids
Aromatic (FWY)	0.128	0.105	0.23	0.34	0.138	0.097	0.39	0.15
Charged (DEKR)	0.128	0.220	-0.65	2.0 x 10−3	0.100	0.225	-0.96	6.9 x 10−5
Hydrophobic (ILMV)	0.291	0.100	1.30	1.7 x 10−10	0.300	0.128	1.07	3.5 x 10−7
Polar (GHST)	0.287	0.313	-0.12	0.50	0.263	0.297	-0.17	0.41
QN	0.027	0.120	-1.59	3.8 x 10−6	0.038	0.117	-1.22	5.1 x 10−4

^ǂ Odds ratios (OR_A) were calculated as in Eq 1, and represent the degree of overrepresentation or underrepresentation of each amino acid among constitutive ADE⁺ isolates.