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. 2018 May 8;18(1):16–24. doi: 10.3892/mmr.2018.8987

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Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Elevated expression levels of SMAD4 are observed in human cataract lens posterior capsular samples and in TGF-β2 induced cataract cell models. SMAD4 was overexpressed in tissue specimens with cataract as detected by (A) immunohistochemistry, (B) RT-qPCR and (C) western blotting, **P<0.01 vs. non-cataract group. (D) A significant positive correlation between KCNQ1OT1 and SMAD4 as presented by Spearman correlation analysis. SMAD4 expression levels were elevated in SRA01/04 cells in the presence of TGF-β2 as detected by (E) RT-qPCR and (F) western blotting, **P<0.01 vs. control group. All data were presented as the mean ± standard deviation from three independent experiments. ID, identification; KCNQ1OT1, potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; SMAD4, mothers against decapentaplegic homolog 4; TGF-β2, transforming growth factor-β2.