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. 2018 May 8;18(1):16–24. doi: 10.3892/mmr.2018.8987

Figure 3.

Figure 3.

Downregulation of KCNQ1OT1 suppresses the proliferation and EMT of TGF-β2-treated SRA01/04 cells. (A) KCNQ1OT1 expression was suppressed by transfection with KCNQ1OT1 shRNA as confirmed by reverse transcription-quantitative polymerase chain reaction, **P<0.01 vs. NC shRNA group. Suppression of KCNQ1OT1 expression inhibited SRA01/04 cell proliferation as determined by (B) Cell Counting Kit-8 and (C) EdU assays, **P<0.01 vs. NC shRNA group. (D) TGF-β2 induced an elevation of fibronectin but a decrease in E-cadherin expression levels (promotion of EMT), and the tendency was reversed by the suppression of KCNQ1OT1, as detected by western blotting, **P<0.01. All data were present as the mean ± standard deviation from three independent experiments. EdU, 5-Ethynyl-20-deoxyuridine; KCNQ1OT1, potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1; NC, negative control; shRNA, short hairpin RNA; TGF-β2, transforming growth factor-β2.