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. 2018 May 9;18(1):322–332. doi: 10.3892/mmr.2018.9002

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — α-synuclein can induce the apoptosis of astrocytes. Apoptosis was measured by a flow cytometry assay. (A) Apoptosis was observed in 4 groups: The control group, the α-syn-WT transfected group, the α-syn-A30P transfected group and the α-syn-A53T transfected group. (B) Statistical analysis of the percentage of apoptotic cells. (C) Cell lysates were prepared for western blot analysis of CHOP levels. (D) Integrated density values for CHOP relative to β-actin. (E) Cell lysates were prepared for western blot analysis of pro-caspase-3 levels. (F) Integrated density values for pro-caspase-3 relative to β-actin. The bars represent mean values ± standard deviation. *P<0.05 vs. FUIGW (negative control). α-syn, α-synuclein; WT, wild-type; HA, influenza A hemagglutinin; GFP, green fluorescence protein; CHOP, CCAAT-enhancer-binding protein homologous protein.