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. 2018 May 9;18(1):349–357. doi: 10.3892/mmr.2018.8998

Figure 5.

Figure 5.

Effect of ANXA1 on apoptosis-associated proteins. (A) The activity of active caspase-3 was assessed by ELISA. (B) Reverse transcription-quantitative polymerase chain reaction was used to test the mRNA expressions of cyclin D1, Bax and Bcl-2. (C) Western blotting was performed to (D) evaluate the protein expressions of cyclin D1, Bax and Bcl-2. The cells were treated with 64 µM Bap for 6 h to establish the cell injury model. *P<0.05 and **P<0.01 vs. control group; ##P<0.01 vs. E.V.+Bap group. ANXA1/Anx, Annexin A1; Bax, Bcl-2-associated X protein; Bcl-2, B-cell lymphoma 2; Bap, benzo[a]pyrene; E.V., empty vector.