Figure 7.

SF1670 reverses the effect of ANXA1. (A) The levels of apoptosis were estimated by flow cytometry assay. (B) Western blotting was performed to determine the expressions of PTEN, FAK, PI3K/Akt, p-PI3K and p-Akt. The cells were treated with 64 µM Bap for 6 h to establish the cell injury model. *P<0.05 and **P<0.01 vs. control group; ^#P<0.05 and ^##P<0.01 vs. E.V.+Bap group; ^P<0.05, ^^P<0.01 vs. ANXA1+BaP group. ANXA1/Anx, Annexin A1; PTEN, phosphatase and tensin homolog; FAK, focal adhesion kinase; PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; p-, phosphorylated; Bap, benzo[a]pyrene; E.V., empty vector.