Skip to main content
. 2018 May 16;18(1):715–722. doi: 10.3892/mmr.2018.9022

Figure 3.

Figure 3.

Effect of PYGB siRNA on cell apoptosis and the cell cycle in the osteosarcoma cell lines MG63 and HOS. (A) Cell apoptosis in MG63 cells was evaluated using flow cytometry following the transfection of PYGB siRNA for 48 h. Early apoptotic cells were identified using Annexin V/propidium iodide double staining and are shown in the lower right quadrant. (B) Cell apoptosis induced by PYGB siRNA in HOS cells was also identified by flow cytometry. (C) The cell cycle distribution of MG63 cells following 48 h post-transfection with PYGB siRNA was detected using flow cytometry. (D) The cell cycle distribution of HOS cells was also evaluated using flow cytometry. *P<0.05 and ***P<0.001 vs. the NC group. Data are expressed as the mean ± standard deviation (n=3). PYGB, Brain type glycogen phosphorylase; NC, negative control; siRNA, small interfering RNA.