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. 2018 May 16;18(1):715–722. doi: 10.3892/mmr.2018.9022

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Effect of PYGB siRNA on the invasion and migration of osteosarcoma cell lines MG63 and HOS. (A and B) The invasive ability of MG63 and HOS cells was evaluated using a Transwell assay following 48 h post-transfection with PYGB siRNA. A smaller number of invaded MG63 and HOS cells were observed and counted in the PYGB siRNA group when compared with the control and NC groups. (C and D) Migrated MG63 and HOS cells were also identified by Transwell assay (magnification, ×200). **P<0.01 and ***P<0.001 vs. the NC group. Data are expressed as the mean ± standard deviation (n=3). PYGB, Brain type glycogen phosphorylase; NC, negative control; siRNA, small interfering RNA.