Effect of HMP on Nrf2, HO-1 and NQO1 protein expression levels in donated after cardiac death rat liver models. (A and B) Following reperfusion, the protein expression levels of Nrf2, HO-1 and NQO1 in the livers of the control, CS and HMP groups were analyzed by western blotting. β-actin served as an internal control. #P<0.05, ##P<0.01 vs. the control group, *P<0.05, **P<0.01 vs. the CS group. (C and D) Western blotting and the quantitative analysis revealed the expression levels of the components associated with the Nrf2-ARE pathway in the livers of the CS and HMP groups prior to and following reperfusion. #P<0.05, ##P<0.01, the CS group vs. the BR CS group; and HMP group vs. the BR HMP group, respectively. AR, after reperfusion; BR, before reperfusion; CS, cold storage; HMP, hypothermic machine pressure; HO-1, heme oxygenase-1; NQO1, NAD(P)H:quinine oxidoreductase 1; Nrf2, nuclear factor erythroid 2-related factor 2.