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. 2018 May 16;18(1):920–930. doi: 10.3892/mmr.2018.9026

Table I.

Oligonucleotide primers used for polymerase chain reaction.

Primer Sequence Product size (bp) Tm
MicroRNA-181a F: 5′-AACATTCAACGCTGTCGGTGAGT-3′ 118 62°C
R: 5′-CTCCTTAGAATCTGTTTGCTCTCATA-3′
U6 F: 5′-CTCGCTTCGGCAGCACA-3′   88 60.6°C
R: 5′-AACGCTTCACGAATTTGCGT-3′

bp, base pairs; F, forward; R, reverse; Tm, primer melting temperature.