Antitumor growth effect on SGC-7901 cells in single and combination treatment therapy as determined by CCK-8 assay. Dark cytotoxicity and antitumor growth effect on SGC-7901 cells by (A) AlPcS4 + 5-FU, (B) AlPcS4 + CDDP, (C) AlPcS4 + DOX, (D) AlPcS4 + MMC or (E) AlPcS4 + VCR. The cells were treated with 1–32 µm/ml free-AlPcS4 or AlPcS4 + 5-FU (20 µm), AlPcS4 + CDDP (5 µm), AlPcS4 + DOX (0.4 µm/ml), AlPcS4 + MMC (0.5 µm/ml) or AlPcS4 + VCR (0.1 µm/ml) for 6 h. The cells were then incubated again for 24 h with or without 635-nm laser irradiation at 100 mW/cm2 illumination dosage for 5 min. *P<0.05, represents a statistical difference in antitumor effect between the combination of AlPcS4 with a chemical agent and free-AlPcS4. AlPcS4, Al(III) phthalocyanine chloride tetrasulfonic acid; 5-FU, 5-fluorouracil; DOX, doxorubicin; CDDP, cisplatin; MMC, mitomycin C; VCR, vincristine.