Figure 4.

Apoptosis induced by AlPcS₄ + 5-FU, AlPcS₄ + CDDP, AlPcS₄ + DOX, AlPcS₄ + MMC, AlPcS₄ + VCR, and free-AlPcS₄ in SGC-7901 cells after being irradiated for 12 h. The cells were treated with 1–32 µm/ml free-AlPcS₄ or AlPcS₄ + 5-FU (20 µm), AlPcS₄ + CDDP (5 µm), AlPcS₄ + DOX (0.4 µm/ml), AlPcS₄ + MMC (0.5 µm/ml) or AlPcS₄ + VCR (0.1 µm/ml) for 6 h. The cells were then irradiated with 635-nm laser irradiation at 100 mw/cm² illumination dosage for 5 min, incubated for 12 h, stained with Hoechst 33342 probe, and then imaged using afluorescence microscope. All the Hoechst staining images were acquired at an ×400 magnification. The scale bar represented 20 µm. AlPcS₄, Al(III) phthalocyanine chloride tetrasulfonic acid; 5-FU, 5-fluorouracil; DOX, doxorubicin; CDDP, cisplatin; MMC, mitomycin C; VCR, vincristine.