Figure 1.

Clinical relevance of c-Met and Jagged1 expression in gastric cancer tissues. (A) Approximately 91 gastric cancer tissues were collected for immunohistochemical staining of c-Met and Jagged1. Patients with positive c-Met expression had poorer overall survival than patients without c-Met expression (P=0.043). (B) Statistical correlation between c-Met and Jagged1 expression was compared using the linear regression model (P=0.004). (C) Effect of HGF and NS398 on the growth of SC-M1/HA-N1IC and SC-M1/pcDNA3 cells. Transfected SC-M1 cells were cultured in the presence or absence of HGF (0, 5 or 25 ng/dl) and NS398 (0 or 50 µM) for 3 days, and the cell growth was measured by MTT assay. Representative data are the average of 6 replicates. (HGF dosage: -, +, and ++ denotes 0, 5, and 25 ng/dl, respectively; NS398 dosage: - and + denotes 0 and 50 µM, respectively; *P<0.01 at 72 h). HGF, hepatocyte growth factor; N1IC, Notch1 intracellular domain.