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. 2018 May 16;40(1):294–302. doi: 10.3892/or.2018.6447

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Copyright: © Huang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Migration analysis in SC-M1/HA-N1IC and SC-M1/pcDNA3 cells. (A) The migration of transfected SC-M1 cells was evaluated in the presence or absence of HGF (25 ng/ml), and/or NS398 (50 µM) in SC-M1/pcDNA and SC-M1/HA-N1IC cells for 24 h. The migration ability of (a) SC-M1/pcDNA3 (b) SC-M1/pcDNA + HGF (c) SC-M1/pcDNA3 + NS398 (d) SC-M1/pcDNA3 + HGF and NS398. (e) SC-M1/HA-N1IC (f) SC-M1/HA-N1IC + HGF (g) SC-M1/HA-N1IC + NS-398 (h) SC-M1/HA-N1IC + HGF and NS398. (B) Migration of transfected SC-M1 cells was evaluated following treatment with HGF (0 and 25 ng/ml), with/without NS-398 (50 µM) in SC-M1/pcDNA3 and SC-M1/HA-N1IC cells for 24 h. Migration ability of (a) SC-M1/pcDNA3 cells, (b) SC-M1/pcDNA3 + HGF, (c) SC-M1/pcDNA3 + NS398, (d) SC-M1/pcDNA3 + HGF and NS398; (e) SC-M1/HA-N1IC cells, (f) SC-M1/HA-N1IC + HGF, (g) SC-M1/HA-N1IC + NS398, (h) SC-M1/HA-N1IC + HGF and NS398. Fold changes in migration of gastric cancer cells from respective groups. Cells were cultured in serum-free media for 24 h before the Transwell assay. SC-M1/pcDNA3 or SC-M1/HA-N1IC cells were treated with/without HGF in the presence/absence of COX-2 inhibitor NS398 for 24 h, and then the migrated cells were counted. Total cell number were counted from nine fields. *P<0.01 compared to SC-M1/pcDNAs. (Data are presented as the mean ± SD of 2 replicates from 3 experiments). N1IC, Notch1 intracellular domain; HGF, hepatocyte growth factor.