Western blot analysis of the protein expression of c-Met, Jagged1 and COX-2 in SC-M1 cells. After HGF (25 ng/dl) treatment at the indicated times (1, 2 or 4 h), the cells were collected, lysed and analyzed for c-Met, Jagged1 and COX-2 expression. (A) Jagged1 and c-Met expression in SC-M1/wild-type (WT), SC-M1/pcDNA3 and SC-M1/HA-N1IC cells. Jagged1 expression in SC-M1/WT and SC-M1/pcDNA3 cells was lower than that in SC-M1/HA-N1IC cells. The expression of c-Met protein was relatively low in SC-M1/HA-N1IC cells. (B) HGF/c-Met signaling induced Jagged1 expression in SC-M1/pcDNA3 cells in a time-dependent manner. (C) HGF/c-Met signaling induced COX-2 expression of SC-M1/pcDNA3 cells in a time-dependent sequence. HGF, hepatocyte growth factor; N1IC, Notch1 intracellular domain.