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. 2018 May 2;40(1):61–72. doi: 10.3892/or.2018.6413

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Copyright: © Fan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — BMF is a direct target of miR-125b in ESCC cancer cells. (A) The prediction of the binding between miR-125b and BMF as determined using TargetScan. (B) A dual-luciferase reporter assay was performed to verify the binding of miR-125b with BMF. (C) qRT-PCR assay was performed to detect the mRNA level of BMF in EC109 and EC9706 cells treated with miR-125b mimics and miR-125b inhibitors. (D) The expression of BMF was assessed in the tumor sections. *P<0.05 vs. the control. BMF, BCL-2-modifying factor; ESCC, esophageal squamous cell carcinoma.