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. 2016 Oct 21;5(10):e16166. doi: 10.1038/lsa.2016.166

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Copyright © 2016 The Author(s)

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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SDOM imaging of actin in fixed mammalian cells. Wide-field image (a) and SDOM image (b) of Alexa Fluor 568 phalloidin-labeled actin in fixed mouse kidney tissue cells. The diagram in b shows the intensity profile of the corresponding line indicated in a, b. (c, e) are zoomed-in wide-field orientation mapping images and (d, f) are zoomed-in SDOM images. Scale bar: (a, b) 2 μm, (c–f) 200 nm.