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. Author manuscript; available in PMC: 2018 Dec 4.
Published in final edited form as: Nat Mater. 2018 Jun 4;17(8):732–739. doi: 10.1038/s41563-018-0099-0

Figure 1. Microgels for controlled presentation of immunomodulatory proteins.

Figure 1

(a) Flow focusing microfluidics were used to generate biotinylated microgels from biotin-functionalized PEG-4MAL macromers. SA-FasL was immobilized on microgels and these immunomodulatory microgels were co-transplanted with islets under the kidney capsule of diabetic mice inducing graft acceptance. (b,c) Biotinylated microgels capture and display SA in a dose-dependent manner until reaching saturation (scale bar 200 µm). Representative experiment (n=3 samples, mean ± SD) shown from 3 independent runs with consistent results. (d) SA-FasL presented on microgels maintains bioactivity and induces dose-dependent apoptosis in FasL-sensitive cells. Representative experiment (n=3 samples, mean ± SD) shown from 3 independent runs with consistent results.