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. 2018 May 4;7:18005. doi: 10.1038/lsa.2018.5

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Copyright © 2018 The Author(s)

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High-resolution, high-accuracy polarization-resolved SHG measurements. Abbreviations: BPF, bandpass filter of 514/3 nm for SHG in the transmission and 620/52 nm for 2PL in the epi-detection; C, condenser, 1.4NA; D or M, dichroic (for 2PL) or silver mirror (for PSHG), both at 45° GM, silver-coated galvanometric mirrors; HWP, zero-order half-waveplate; L1,2, achromatic lenses; LP, linear polarizer; M, silver mirror; O, objective 40 ×, 1.3NA; PMT1,2, photomultiplier tubes; SP, sample plane; SPF, short-pass filter. The excitation linear polarization starts horizontal in the SP and is rotated clockwise with an angle φ. Typically, φ is rotated 0°–360° with a step of 1°.