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. 2018 Jul 25;9:2908. doi: 10.1038/s41467-018-05374-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Analysis of a partial knock-down of mosGILT and the impact on sporozoite cell traversal (a) RT-qPCR of SGs injected with either dsRNA targeting an irrelevant luciferase gene or mosGILT. Data represent duplicate experiments from more than three experiments (mean ± SD, unpaired t-test, ***p < 0.0005). b Western blots of SGs from dsluc or dsmosGILT injected mosquitoes. c Cell traversal assay of murine hepatic cells incubated with P. berghei sporozoites isolated from dsluc or dsmosGILT salivary glands (2000 sporozoites/well). Cell traversal by sporozoites was analyzed on a Stratedigm flow cytometer by measuring the percentage of fluorescent dextran positive cells. Each point represents a technical replicate pooled from two independent experiments (n = 7, ns not significant)