Figure 2.

Skeletal muscle mRNA and protein abundance of autophagy markers in mice. (A) Twelve weeks of a high‐fat diet (HFD) lowered LC3 mRNA abundance compared to a low‐fat diet (LFD), with no effect of clamp condition. (B) HFD lowered p62 mRNA abundance compared to LFD, with no effect of clamp condition. (C) LC3II/LC3I protein abundance was suppressed in the HI/HG clamp. (D) p62 protein abundance was not changed by diet or clamp conditions. (E) Representative immunoblots with molecular weight markers (MWM). All samples were derived at the same time and processed in parallel. Total mRNA was extracted from quadriceps and relative quantification performed by quantitative polymerase chain reaction. Data were analyzed by two‐way ANOVA corrected with Tukey's multiple comparison test, *P < 0.05 compared to SAL, **P < 0.05 compared to HI/EG, ***P < 0.05 compared to SAL and HI/EG. Data are expressed as means and SD, n = 7–10 per group. AU, arbitrary units.