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. 2018 Jul 25;6(14):e13807. doi: 10.14814/phy2.13807

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© 2018 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The effect of chemogenetic activation of DMH to PAG projections on thermoregulation. (A) Rats were implanted with an intraabdominal transmitter with one lead to the liver (to measure core body temperature) and one lead to brown adipose tissue (BAT). To selectively study the effect of dorsomedial hypothalamus (DMH) to periaqueductal grey (PAG) projections on thermoregulation, CAV2Cre was injected into the PAG and Cre‐dependent DREADD hM ₃D(G_q) mCherry was injected into the DMH. An AAV‐hSyn‐YFP virus was injected together with CAV2Cre to visualize the injection site in the PAG. (B) Example injection site of DREADD hM ₃D(G_q) in the DMH, showing hM3D(Gq)‐mCherry‐positive neurons in the DMH and surrounding areas. (C) CNO treatment in rats with hM3D(Gq)‐mCherry‐positive neurons in the DMH increased core body temperature, BAT temperature, and locomotor activity. Data are shown as mean ± SEM; n = 7–11.