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. 2018 Jul 10;4:65. doi: 10.1038/s41420-018-0067-0

Fig. 8. Oxa12 reduces NF-κB/IκB ratio and NF-κB p65 nuclear translocation when compared to zVAD-fmk-treated cells.

Fig. 8

a BV2 cells were incubated with zVAD-fmk (25 μM) in the presence or absence of Nec-1 (30 µM) or Oxa12 (30 µM) for 24 h. Representative immunoblots of total NF-κB are presented together with the respective densitometric analysis of NF- κB/IκB ratio. β-actin was used as loading control. Results are expressed as mean ± SEM from three independent experiments. *p < 0.05 vs. control; ǂp < 0.05 vs. zVAD-fmk. b Representative images of immunofluorescence staining showing NF-κB p65 (red) nuclear translocation in BV2 cells treated with zVAD-fmk (25 µM) in the presence or absence of Nec-1 (30 µM) or Oxa12 (30 µM) for 5 h, and quantification of mean fluorescence intensity. Cell nuclei were detected by Hoechst (blue). Scale bar=1 µm