Table 2.
Oligos used in the studies.
| Target site | Application | Forward oligo (5′ > 3′) | Reverse oligo (5′ > 3′) |
|---|---|---|---|
| mNap1l3* | qPCR | ||
| mHprt1** | qPCR | ||
| hNAP1L3 | qPCR | ACCAGAGGTGAAAGCTGAA | CCTGGGGGACTTCTTTAGGA |
| hUBC | qPCR | CTGGAAGATGGTCGTACCCTG | GGTCTTGCCAGTGAGTGTCT |
| hHOXA3 | qPCR | CTCCAGCTCAGGCGAAAG | ACAGGTAGCGGTTGAAGTGG |
| hHOXA4 | qPCR | CATGTCAGCGCCGTTAACC | ACCTGCTGCCGGGTGTAG |
| hHOXA5 | qPCR | CGCCCAACCCCAGATCTAC | CCGCCTATGTTGTCATGACTTATG |
| hHOXA6 | qPCR | CGCGGGTGCTGTGTATG | CCTTCTCCAGCTCCAGTGTC |
| hHOXA9 | qPCR | CCCCATCGATCCCAATAACC | CCAGGGTCTGGTGTTTTGTATAGG |
| hCDK1 | qPCR | GGAAACCAGGAAGCCTAGCA | GATCATAGATTAACATTTTCGAGAGCAA |
| mNAP1l3 | OE cloning | ANGCTCTAGAATGGCAGAAGCGGATCCTAAAATG | ANGCGCGGCCGCCTACTTGTAGTACTTCCTATTT CATAAT |
| mNAP1l3 | CRISPR gRNA | ACCGGCGCCTCTTCAGCAACCCCAT | AAACATGGGGTTGCTGAAGAGGCGC |
| mNAP1l3 | PCR mutation analysis | CCACAGCTGCTGCAGTCC | GCCCTTCTGGAAGGCTCAG |
| mNAP1l3 | Sequencing mutation analysis | CAGTCCGTGTTGCCACTG |
*mNap1l3 Taqman probes was purchased from ThermoFisher Scientific (Mm01214875_s1).
**mHrpth Taqman probes was purchased from ThermoFisher Scientific (Mm03024075_m1).