Skip to main content
. 2018 Jul 9;4:74. doi: 10.1038/s41420-018-0074-1

Fig. 2. Loss of function of Pont triggers JNK-mediated cell death in the larval wing discs.

Fig. 2

Fluorescent images of acridine orange staining of the third-instar larva wing disc (a–c) and β-galactosidase immunostaining for puc-LacZ (d–f) are shown. Knockdown of pont in the posterior compartment of the wing disc driven by en-Gal4 (a, d) triggered cell death (b) and puc activation (e), which were suppressed by expressing of Puc (c, f). The lower panels are the magnification of the boxed area in the upper panels. g Statistical analysis of acridine orange-positive cells in (a–c). Error bars means ± SEM, ***P ≤ 0.001. Scale bar for a–c, 200 μm. Scale bar for d–f, 100 μm. Genotypes en-Gal4/+ (a); en-Gal4/UAS-pont-IR #2 (b); en-Gal4 UAS-pont-IR #2/+; UAS-Puc/+ (c); en-Gal4/+; pucE69/+ (d); en-Gal4/UAS-pont-IR #2; pucE69/+ (e); en-Gal4 UAS-pont-IR #2/+; pucE69/UAS-Puc (f)