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. 2018 Jul 25;8:11206. doi: 10.1038/s41598-018-29156-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PD-L1 expression in pleural tissue (PT), pleural effusions (PE) of pulmonary adenocarcinoma by immuhistochemical stain (400 times magnification). The slices of (a–f) are matched respectively by PT and PE from the same patients. If 5 points is the cut-off value, PD-L1 expression of PT and PE are both positive in the first case, a negative and a positive in the second case, both negative in the third case. The (g and h) are positive (lymphoma) and negative (breast carcinoma) control. The values in brackets are the mean scores in each slice of five scopes.