Figure 3. H₂S activated TRPV1-mediated Ca²⁺ influx.

a NaHS treatment could induce Ca²⁺ influx in DPSCs, as assessed by Ca²⁺ levels using the ratio of emission in response to excitation at 340 and 380 nm on an Olympus Optical IX71 microscope. b NaHS treatment could induce Ca²⁺ influx in DPSCs as capsaicin treatment. c Capsaicin, but not NaHS, could induce Ca²⁺ influx in CBS siRNA-pre-treated DPSCs. d Capsaicin, but not NaHS, could induce Ca²⁺ influx in CSE siRNA-pre-treated DPSCs. e Both capsaicin-induced and NaHS-induced Ca²⁺ influx was blocked by capsazepine treatment. f Knockdown of TRPV1 by siRNA could partially block the Ca²⁺ influx induced by NaHS treatment. Caps capsaicin. All experimental data were verified in at least three independent experiments