Fig. 3.

Peripheral T lymphocytes enter cell cycle after 1 week of refeeding. Calorically restricted mice were returned to AL food access and treated with BrdU in their drinking water. The T cell compartment was analyzed by flow cytometry. a Absolute count of T cells per μl of blood. b Absolute count of Ki67+BrdU+ CD8a and c CD4 T cells. d–f Absolute count of Ki67+BrdU+ central memory (CD44+CD62L+), naïve (CD44−CD62L+), and effector memory (CD44+CD62L−) cells in blood. Statistical differences were calculated by two-tailed Mann Whitney U tests by comparing cellularity to age-matched, AL fed mice. *P < 0.05. Data are representative of two independent experiments with 3–7 mice per treatment group