Fig. 4.

Lymphoid organ cellularity returns to AL levels following 2 months of refeeding. Calorically restricted mice were maintained on AL food for 2 months after CR treatment. Lymphocyte subsets were then analyzed by flow cytometry from the a spleen, b blood, c brachial and inguinal lymph nodes, and d bone marrow of the femur. Statistical differences were calculated by two-tailed Mann Whitney U tests by comparing cellularity to age-matched, AL fed mice. We found no statistical difference in numbers between the groups. Data are representative of three independent experiments with 2–8 mice per treatment group