Fig. 5.

Thymic cellularity returns to AL levels following 2 months of refeeding. Calorically restricted mice were maintained on AL food for 2 months after CR treatment. Thymus subsets were analyzed by flow cytometry. a Absolute counts of CD4 single positive, CD8 single positive, double negative, and CD4/CD8 double positive thymocytes. b Absolute count of CD4/CD8 double negative thymocyte subsets, based on CD44 and CD25 expression. Statistical differences were calculated by two-tailed Mann Whitney U tests by comparing cellularity to age-matched, AL fed mice. *P < 0.05. Data are representative of three independent experiments with 2–8 mice per treatment group