Fig. 6.

Thymectomized mice display CR-induced lymphopenia. B6 mice were thymectomized in house or at the Jackson Laboratory (Bar Harbor, ME) and then subjected to 2 months of CR and lymphocyte subsets were analyzed by flow cytometry from the a spleen, b blood, c brachial and inguinal lymph nodes, and d bone marrow of the femur. Statistical differences were calculated by one-tailed Mann Whitney U tests by comparing cellularity to age-matched, AL fed mice. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Data are representative of two independent experiments with 4–8 mice per treatment group