Skip to main content
. 2018 Jul 19;9:889. doi: 10.3389/fphys.2018.00889

FIGURE 4.

FIGURE 4

SPL-mediated increase in AE2 activity was dependent on the intracellular Ca2+. (A) The effect of free Ca2+ chelator BAPTA-AM (BAPTA, 10 μM, 1 h) on SPL-mediated CBE activity and Cl− transporting activity of AE2, respectively, in HEK293T cells. (B) Bars indicate the means ± SEM of the number of experiments. The effect of BAPTA on AE2 with SPL was normalized to the activity of the control (black). (∗P < 0.05, n = 5). (C) Cl− transporting activity with MQAE technique in AE2 with or without SPL in HEK293T cells. (D) Bars indicate the means ± SEM of the number of experiments (∗P < 0.05, n = 4). (E) The interaction between AE2 and SPL with and without BAPTA-AM in HEK293T cells. The brackets represent the detected antibodies. (F) The effect of BAPTA-AM (10 μM) for 1 h on CBE activities of isolated SMG acini and ductal cells. (G) Bars indicate the means ± SEM of the number of experiments (∗P < 0.05, n = 4). (H) Changes Cl− transporting activity by BAPTA-AM (10 μM) with MQAE quenching technique in isolated SMG ductal cells. (I) Bars indicate the means ± SEM of the number of experiments (∗P < 0.05, n = 3).