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. 2018 Jul 19;9:889. doi: 10.3389/fphys.2018.00889

FIGURE 6.

FIGURE 6

Microtubule stabilization maintain the ductal CBE activity. (A) The interaction between AE2 and SPL with and without nocodazole (10 μM, 1 h) in HEK293T cells. The brackets represent the detected antibodies. β-actin was used as a loading control. (B) Changes in pHi of isolated SMG ductal cells with and without nocodazole (Noco). (C) Bars indicate the means ± SEM of the number of experiments (n = 4). NS, not stipulated. (D) Changes Cl− transporting activity by nocodazole (10 μM) with MQAE quenching technique in isolated SMG ductal cells. (E) Bars indicate the means ± SEM of the number of experiments (∗P < 0.05, n = 3).