Table 2.
Composition of HEPES-based solution.
| Composition | Concentration |
|---|---|
| Sodium chloride (NaCl) | 140 mM |
| HEPES | 10 mM |
| Glucose | 10 mM |
| Potassium chloride (KCl) | 5 mM |
| Magnesium chloride (MgCl2) | 1 mM |
| Calcium chloride (CaCl2) | 1 mM |
| pH 7.4 | |
| 300 mOsm (310 for SMG) | |
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