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. 2018 Jul 19;9:1562. doi: 10.3389/fimmu.2018.01562

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Copyright © 2018 Sheng, Qian, Tang, Xing and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transepithelial transport of the OVA evidenced by IEM. (A–C) The distribution of the OVA-positive gold particles in various sized vesicles, tubules, and multivesicular bodies in the cytoplasm, around the surface microvilli of IECs and in gut lumen in group 1 at 2 h after challenge. (D–F) The distribution of the OVA-positive gold particles in IEC of group 1 at 9 h after challenge. (G–I) No gold particles were detected in negative control staining (G) and IECs of hindgut in group 2 (H,I) at 2 h after challenge. Abbreviations: v, vesicles; tv, tubule vesicles; mvb, multivesicular bodies; mv, microvilli; IECs, intestinal epithelial cells; OVA, ovalbumin; IME, immunogold electron microscopy.