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. 2018 Jul 19;9:1562. doi: 10.3389/fimmu.2018.01562

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Copyright © 2018 Sheng, Qian, Tang, Xing and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Co-localization of pIgR, IgM, and OVA in hindgut of flounder. Pictures from group 1 were shown as examples. The fish immunized intraperitoneally with OVA and subsequently challenged with OVA via caudal vein on the fourth week after immunization, and pIgR, IgM, and OVA in hindgut was stained at 2 h after challenge, presenting orange, green and red fluoresce respectively. The cell nucleus was counterstained in blue with DAPI. (A,B,C) Distribution of IgM, OVA, pIgR in hindgut at 2 h after challenge was illuminated, respectively. (D–F) The merged picture of (A,B,G) and (A,C,G) and (B,C,G), respectively. (G) The cell nucleus of intestinal epithelium was stained blue by DAPI. (H) The merged picture of (A–C,G); (I) Negative control. Bar = 25 μm. Abbreviations: LP, lamina propria submucosa; Lu, lumen; IECs, intestinal epithelial cells; Mu, mucus cells; BV, blood vessel; OVA, ovalbumin.