FIGURE 2.

PilT protein-based maximum likelihood phylogeny. Myxobacterial pilT encoded proteins were subjected to BLASTp against the studied organisms. The top hits were extracted and aligned with myxobacterial homologs, which we further used for the construction of the phylogenetic tree. Myxobacterial and outgroup pilT homologs, which are a part of a T4aP cluster, are shaded throughout the full clade from the center. Bootstrap values (>50%) are depicted here adjacent to the tree nodes. In the outer circle, we mapped taxonomy according to color codes shown in the left corner. Deltaproteobacteria-I includes members of orders Bdellovibrionales and Desulfuromonadales whereas Deltaproteobacteria-II includes members of Bradymonadales, Desulfarculales, Desulfobacterales, Desulfurellales, Desulfovibrionales, and Syntrophobacterales.